Development & Validation of Stability Indicating High Performance Liquid Chromatographic Method for Simultaneous Estimation of Atenolol & Indapamide in Tablet Dosage Form

In the present study a simple, accurate & precise reverse phase liquid chromatographic method has been developed & validated for simultaneous estimation of Atenolol & Indapamide from tablet dosage form. The method was developed using Waters HPLC system on a L1 column (Hypersil Gold: 250mm x 4.6 mm, 5μm) using a mixture of 0.1% Triethyl Amine in water of pH 3.0 & Methanol in the ratio 30:70 v/v as mobile phase in an isocratic elution mode at a flow rate of 1.0 ml/min, at 30oC with a load of 20μl. The detection was carried out at 240 nm. The retention time of Atenolol & Indapamide were found to be around 3.05 min & 3.93 min respectively. The method was validated with respect to linearity, robustness, precision, specificity & accuracy. The proposed method was successfully applied for the simultaneous quantitative determination of Atenolol & Indapamide from the tablet dosage form.